Virulence profiles of Shiga toxin-producing Escherichia coli and other potentially diarrheagenic E.coli of bovine origin, in Mendoza, Argentina
Braz. j. microbiol
;
44(4): 1173-1180, Oct.-Dec. 2013. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-705281
ABSTRACT
This study described a group of strains obtained from a slaughter house in Mendoza, in terms of their pathogenic factors, serotype, antibiotype and molecular profile. Ninety one rectal swabs and one hundred eight plating samples taken from carcasses of healthy cattle intended for meat consumption were analyzed. Both the swab and the plate samples were processed to analyze the samples for the presence of virulence genes by PCR stx1, stx2, eae and astA. The Stx positive strains were confirmed by citotoxicity assay in Vero cells. The isolates were subsequently investigated for their OH serotype, antimicrobial susceptibility and molecular profile by Random Amplification of Polymorphic DNA (RAPD). Twelve E.coli strains were identified by their pathogenicity. Nine were from fecal origin and three from carcasses. Three strains carried the stx1 gene, three the stx2 gene, two carried eae and four the astA gene. The detected serotypes were O172H-; O150H8; O91H21; O178H19 and O2H5. The strains showed a similarity around 70% by RAPD. Some of the E.coli strains belonged to serogroups known for certain life-threatening diseases in humans. Their presence in carcasses indicates the high probability of bacterial spread during slaughter and processing.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
État de porteur sain
/
Facteurs de virulence
/
Infections à Escherichia coli
/
Escherichia coli producteur de Shiga-toxine
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
Pays comme sujet:
Amérique du Sud
/
Argentine
langue:
Anglais
Texte intégral:
Braz. j. microbiol
Thème du journal:
Microbiologie
Année:
2013
Type:
Article
Pays d'affiliation:
Argentine
Institution/Pays d'affiliation:
Universidad Nacional de Cuyo/AR
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS