Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: temporal and regional trends
Mem. Inst. Oswaldo Cruz
;
109(2): 236-245, abr. 2014. tab, graf
Article
Dans Anglais
| LILACS
| ID: lil-705811
ABSTRACT
Mycobacterium bovis is the causative agent of bovine tuberculosis (TB), a disease that affects approximately 5% of Argentinean cattle. Among the molecular methods for genotyping, the most convenient are spoligotyping and variable number of tandem repeats (VNTR). A total of 378 samples from bovines with visible lesions consistent with TB were collected at slaughterhouses in three provinces, yielding 265 M. bovis spoligotyped isolates, which were distributed into 35 spoligotypes. In addition, 197 isolates were also typed by the VNTR method and 54 combined VNTR types were detected. There were 24 clusters and 27 orphan types. When both typing methods were combined, 98 spoligotypes and VNTR types were observed with 27 clusters and 71 orphan types. By performing a meta-analysis with previous spoligotyping results, we identified regional and temporal trends in the population structure of M. bovis. For SB0140, the most predominant spoligotype in Argentina, the prevalence percentage remained high during different periods, varying from 25.5-57.8% (1994-2011). By contrast, the second and third most prevalent spoligotypes exhibited important fluctuations. This study shows that there has been an expansion in ancestral lineages as demonstrated by spoligotyping. However, exact tandem repeat typing suggests dynamic changes in the clonal population of this microorganism.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Tuberculose bovine
/
Techniques de typage bactérien
/
Techniques de génotypage
/
Mycobacterium bovis
Type d'étude:
Étude pronostique
/
Facteurs de risque
Limites du sujet:
Animaux
Pays comme sujet:
Amérique du Sud
/
Argentine
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
2014
Type:
Article
/
descriptif de projet
Pays d'affiliation:
Argentine
Institution/Pays d'affiliation:
National University of Mar del Plata/AR
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