Evaluation of polymerase chain reaction (PCR) for identifying Mycobacterium bovis isolates from the modified Middlebrook 7H11 agar thin layer technique
Rev. Inst. Adolfo Lutz
;
71(4): 753-756, out.-dez. 2012. tab
Article
Dans Anglais
| LILACS, SES-SP, SESSP-CTDPROD, SES-SP, SESSP-ACVSES, SESSP-IALPROD, SES-SP, SESSP-IALACERVO
| ID: lil-706148
ABSTRACT
The associated use of the modified Middlebrook 7H11 agar thin layer technique and the Polymerase Chain Reaction (PCR) assay enabled to perform the early identification of microcolonies of Mycobacterium bovis from 12th to 25th day of culture. In order to reduce the time for performing the Mycobacterium bovis identification, the combined use of these two techniques was evaluated by analyzing the microcolonies of mycobacteria at the 8th day after culturing. Until the last day of analysis, all of the PCR-positive samples already showed the microcolonies. Therefore, the early diagnosis of bovine tuberculosis is feasible, without an apparent macroscopic colonies growth.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Tuberculose
/
Réaction de polymérisation en chaîne
/
Techniques et procédures diagnostiques
/
Agar-agar
/
Mycobacterium bovis
Type d'étude:
Etude diagnostique
/
Étude de dépistage
langue:
Anglais
Texte intégral:
Rev. Inst. Adolfo Lutz
Année:
2012
Type:
Article
Institution/Pays d'affiliation:
Agência Paulista de Tecnologia dos Agronegócios/BR
/
Universidade de São Paulo/BR
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