Thin layer microcolony culture associated with PCR for early identification of Mycobacterium bovis
Braz. j. microbiol
;
45(1): 225-230, 2014. ilus, tab
Article
Dans Anglais
| LILACS
| ID: lil-709456
ABSTRACT
The initial growth of mycobacteria from 49 samples of cattle and buffalo organs collected in commercial slaughterhouses was compared between modified Middlebrook 7H11 thin layer microcolony culture and Stonebrink medium used in the isolation of Mycobacterium bovis. Aliquots were decontaminated by Petroff's method, processed and cultured in both media. The identity of the acid-fast bacilli stained by Ziehl-Neelsen was confirmed by PCR. Optical microscopy showed that results of the early observation of Mycobacterium bovis colonies in thin layer culture were similar to those obtained in macroscopic observation of the colonies in Stonebrink medium. However, early observation of the colonies enabled early confirmation by PCR, given the shorter time to the visualization of colonies when thin layer culture was used (between the 12nd and 25th day of culture).
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Tuberculose bovine
/
Réaction de polymérisation en chaîne
/
Techniques bactériologiques
/
Techniques de diagnostic moléculaire
/
Viande
/
Mycobacterium bovis
Type d'étude:
Etude diagnostique
/
Études d'évaluation
/
Étude pronostique
/
Facteurs de risque
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Braz. j. microbiol
Thème du journal:
Microbiologie
Année:
2014
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Universidade de São Paulo/BR
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