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Oxidative stress enhances the expression of sulfur assimilation genes: preliminary insights on the Enterococcus faecalis iron-sulfur cluster machinery regulation
Riboldi, Gustavo Pelicioli; Bierhals, Christine Garcia; Mattos, Eduardo Preusser de; Frazzon, Ana Paula Guedes; d?Azevedo, Pedro Alves; Frazzon, Jeverson.
Affiliation
  • Riboldi, Gustavo Pelicioli; Universidade Federal de Ciências da Saúde de Porto Alegre. Departamento de Microbiologia. Laboratório de Cocos Gram-positivos e Microbiologia Molecular. Porto Alegre. BR
  • Bierhals, Christine Garcia; Universidade Federal de Ciências da Saúde de Porto Alegre. Departamento de Microbiologia. Laboratório de Cocos Gram-positivos e Microbiologia Molecular. Porto Alegre. BR
  • Mattos, Eduardo Preusser de; Universidade Federal de Ciências da Saúde de Porto Alegre. Departamento de Microbiologia. Laboratório de Cocos Gram-positivos e Microbiologia Molecular. Porto Alegre. BR
  • Frazzon, Ana Paula Guedes; Universidade Federal de Ciências da Saúde de Porto Alegre. Departamento de Microbiologia. Laboratório de Cocos Gram-positivos e Microbiologia Molecular. Porto Alegre. BR
  • d?Azevedo, Pedro Alves; Universidade Federal de Ciências da Saúde de Porto Alegre. Departamento de Microbiologia. Laboratório de Cocos Gram-positivos e Microbiologia Molecular. Porto Alegre. BR
  • Frazzon, Jeverson; Universidade Federal de Ciências da Saúde de Porto Alegre. Departamento de Microbiologia. Laboratório de Cocos Gram-positivos e Microbiologia Molecular. Porto Alegre. BR
Mem. Inst. Oswaldo Cruz ; 109(4): 408-413, 03/07/2014. graf
Article de En | LILACS | ID: lil-716304
Bibliothèque responsable: BR1.1
ABSTRACT
The Firmicutes bacteria participate extensively in virulence and pathological processes. Enterococcus faecalis is a commensal microorganism; however, it is also a pathogenic bacterium mainly associated with nosocomial infections in immunocompromised patients. Iron-sulfur [Fe-S] clusters are inorganic prosthetic groups involved in diverse biological processes, whose in vivo formation requires several specific protein machineries. Escherichia coli is one of the most frequently studied microorganisms regarding [Fe-S] cluster biogenesis and encodes the iron-sulfur cluster and sulfur assimilation systems. In Firmicutes species, a unique operon composed of the sufCDSUB genes is responsible for [Fe-S] cluster biogenesis. The aim of this study was to investigate the potential of the E. faecalis sufCDSUB system in the [Fe-S] cluster assembly using oxidative stress and iron depletion as adverse growth conditions. Quantitative real-time polymerase chain reaction demonstrated, for the first time, that Gram-positive bacteria possess an OxyR component responsive to oxidative stress conditions, as fully described for E. coli models. Likewise, strong expression of the sufCDSUB genes was observed in low concentrations of hydrogen peroxide, indicating that the lowest concentration of oxygen free radicals inside cells, known to be highly damaging to [Fe-S] clusters, is sufficient to trigger the transcriptional machinery for prompt replacement of [Fe-S] clusters.
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Texte intégral: 1 Indice: LILACS Sujet Principal: Enterococcus faecalis / Stress oxydatif / Ferrosulfoprotéines langue: En Texte intégral: Mem. Inst. Oswaldo Cruz Thème du journal: MEDICINA TROPICAL / PARASITOLOGIA Année: 2014 Type: Article / Project document

Texte intégral: 1 Indice: LILACS Sujet Principal: Enterococcus faecalis / Stress oxydatif / Ferrosulfoprotéines langue: En Texte intégral: Mem. Inst. Oswaldo Cruz Thème du journal: MEDICINA TROPICAL / PARASITOLOGIA Année: 2014 Type: Article / Project document