Evaluation of three enzyme immunoassays and a nucleic acid amplification test for the diagnosis of Clostridium difficile-associated diarrhea at a university hospital in Brazil
Rev. Soc. Bras. Med. Trop
;
47(4): 447-450, Jul-Aug/2014. tab
Article
Dans Anglais
| LILACS
| ID: lil-722303
ABSTRACT
Introduction Despite the known importance of Clostridium difficile as a nosocomial pathogen, few studies regarding Clostridium difficile infection (CDI) in Brazil have been conducted. To date, the diagnostic tests that are available on the Brazilian market for the diagnosis of CDI have not been evaluated. The aim of this study was to compare the performances of four commercial methods for the diagnosis of CDI in patients from a university hospital in Brazil. Methods Three enzyme immunoassays (EIAs) and one nucleic acid amplification test (NAAT) were evaluated against a cytotoxicity assay (CTA) and toxigenic culture (TC). Stool samples from 92 patients with suspected CDI were used in this study. Results Twenty-five (27.2%) of 92 samples were positive according to the CTA, and 23 (25%) were positive according to the TC. All EIAs and the NAAT test demonstrated sensitivities between 59 and 68% and specificities greater than 91%. Conclusions All four methods exhibited low sensitivities for the diagnosis of CDI, which could lead to a large number of false-negative results, an increased risk of cross-infection to other patients, and overtreatment with empirical antibiotics. .
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Clostridioides difficile
/
Techniques immunoenzymatiques
/
Infections à Clostridium
/
Techniques d'amplification d'acides nucléiques
/
Diarrhée
Type d'étude:
Etude diagnostique
/
Études d'évaluation
/
Facteurs de risque
Limites du sujet:
Humains
Pays comme sujet:
Amérique du Sud
/
Brésil
langue:
Anglais
Texte intégral:
Rev. Soc. Bras. Med. Trop
Thème du journal:
Médecine tropicale
Année:
2014
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Universidade Federal de Minas Gerais/BR
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS