Tracking false-negative results in molecular diagnosis: proposal of a triplex-PCR based method for leishmaniasis diagnosis
J. venom. anim. toxins incl. trop. dis
;
2004/02/2014.
Article
Dans Anglais
| LILACS
| ID: lil-724683
ABSTRACT
Molecular biological methods have become increasingly relevant to the diagnosis and control of infectious diseases, such as leishmaniasis. Since various factors may affect the sensitivity of PCR assays, including DNA yield and purity, an optimal extraction method is pivotal. Losses of a parasites DNA during extraction may significantly impair its detection by PCR and lead to false-negative results. This study proposes a triplex PCR assay targeting the parasites DNA, an external control (pUC18) and an internal control (G3PD) for accurate diagnosis of leishmaniasis.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Leishmaniose
/
Réaction de polymérisation en chaîne
/
Diagnostic
/
Faux négatifs
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
J. venom. anim. toxins incl. trop. dis
Thème du journal:
Toxicologie
Année:
2014
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Centro de Pesquisas Aggeu Magalhães/BR
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