Validation of a real-time PCR assay for the molecular identification of Mycobacterium tuberculosis
Braz. j. microbiol
;
45(4): 1362-1369, Oct.-Dec. 2014. graf, tab
Article
Dans Anglais
| LILACS
| ID: lil-741288
ABSTRACT
Mycobacterium tuberculosis is the major cause of tuberculosis in humans. This bacillus gained prominence with the occurrence of HIV, presenting itself as an important opportunistic infection associated with acquired immunodeficiency syndrome (AIDS). The current study aimed to develop a real-time PCR using Eva Green technology for molecular identification of M. tuberculosis isolates. The primers were designed to Rv1510 gene. Ninety nine samples of M. tuberculosis and sixty samples of M. bovis were tested and no sample of the bovine bacillus was detected by the qPCR. Statistical tests showed no difference between the qPCR and biochemical tests used to identify the Mycobacterium tuberculosis. The correlation between tests was perfect with Kappa index of 1.0 (p < 0.001, CI = 0.84 - 1.0). The diagnostic sensitivity and specificity were 100% (CI = 95.94% - 100%) and 100% (CI = 93.98% - 100%). This qPCR was developed with the goal of diagnosing the bacillus M. tuberculosis in samples of bacterial suspension. TB reference laboratories (health and agriculture sectors), public health programs and epidemiological studies probably may benefit from such method.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Tuberculose
/
Réaction de polymérisation en chaine en temps réel
/
Mycobacterium tuberculosis
Type d'étude:
Etude diagnostique
/
Étude pronostique
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Braz. j. microbiol
Thème du journal:
Microbiologie
Année:
2014
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Laboratório Nacional Agropecuário de Minas Gerais/BR
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS