Expression analysis of notsh signaling pathway molecules in shed cultured in keratinocyte growth medium
Braz. j. oral sci
;
14(2): 135-140, Apr.-June 2015. tab, ilus
Article
Dans Anglais
| LILACS
| ID: lil-755036
ABSTRACT
Aim:
To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM).Methods:
RNA was extracted from SHED harvested on day 1, 3 and 7. RNA was reverse-transcribed to obtain the cDNA and then proceeded with PCR using specific primers for the Notch signaling pathway molecules (Notch1, Jagged-1, Jagged-2 and, Hes1) as well as stem cell marker (Nanog). PCR products were electrophoresed on a 2% agarose gel and stained with SYBR green.Results:
Notch-1 was highly expressed in SHED cultured in KGM and showed increase in density as the days progressed, while Jagged-1 showed a decrease. Jagged-2 on the other hand, showed a slight increase on day 3 followed by a decrease on day 7. However, Hes-1 was not expressed in SHED cultured in KGM. Nanog showed expression only on day 3 and gradually increased in expression on day 7.Conclusions:
Notch signaling pathway associated molecules; Notch-1, Jagged-1, Jagged-2, and stem cell marker Nanog are expressed in SHED cultured in KGM which may be involved in the differentiation into epithelial-like cells in human dental pulp tissues.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Cellules souches
/
Dent de lait
/
Expression des gènes
/
Kératinocytes
/
Milieux de culture
/
Récepteurs Notch
Limites du sujet:
Femelle
/
Humains
/
Mâle
langue:
Anglais
Texte intégral:
Braz. j. oral sci
Thème du journal:
Dentisterie
Année:
2015
Type:
Article
/
descriptif de projet
Pays d'affiliation:
Malaisie
Institution/Pays d'affiliation:
International Islamic Universiti Malaysia/MY
/
Universiti Sains Malaysia/MY
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