An extracellular β-agarase was purified from Pseudoalteromonas sp. NJ21, a Psychrophilic agar-degrading bacterium isolated from Antarctic Prydz Baysediments. The purified agarase (Aga21) revealed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with an apparent molecular weight of 80 kDa. The optimum pH and temperature of the agarase were 8.0 and 30 °C, respectively. However, it maintained as much as 85% of the maximum activities at 10 °C. Significant activation of the agarase was observed in the presence of Mg2+, Mn2+, K+; Ca2+, Na+, Ba2+, Zn2+, Cu2+, Co2+, Fe2+, Sr2+ and EDTA inhibited the enzyme activity. The enzymatic hydrolyzed product of agar was characterized as neoagarobiose. Furthermore, this work is the first evidence of cold-adapted agarase in Antarctic psychrophilic bacteria and these results indicate the potential for the Antarctic agarase as a catalyst in medicine, food and cosmetic industries.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Adaptation physiologique
/
Pseudoalteromonas
/
Agar-agar
/
Glycosidases
langue:
Anglais
Texte intégral:
Braz. j. microbiol
Thème du journal:
Microbiologie
Année:
2015
Type:
Article
/
descriptif de projet
Pays d'affiliation:
Suisse
Institution/Pays d'affiliation:
The First Institute of Oceanography/CH
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Adaptation physiologique
/
Pseudoalteromonas
/
Agar-agar
/
Glycosidases
langue:
Anglais
Texte intégral:
Braz. j. microbiol
Thème du journal:
Microbiologie
Année:
2015
Type:
Article
/
descriptif de projet
Pays d'affiliation:
Suisse
Institution/Pays d'affiliation:
The First Institute of Oceanography/CH
Consulta Detalhada
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