Remote limb ischemic post-conditioning attenuates ischemia-reperfusion injury in rat skin flapby limiting oxidative stress
Acta cir. bras
;
31(1): 15-21, Jan. 2016. tab, graf
Article
Dans Anglais
| LILACS
| ID: lil-771850
ABSTRACT
PURPOSE:
To investigate the effect of remote ischemic post-conditioning (RIPoC) against ischemia-reperfusion (I/R) injury on flaps of rats.METHODS:
Sprague-Dawley rats were randomized into the Sham, Control, RIPoC1 and RIPoC2 groups. All the animals were submitted to a 5×4 cm superficial inferior epigastric artery flap. Eight hours of flap ischemia was induced and two protocols of limb RIPoC were applied. Tissue MDA level and SOD activity in 24-h reperfusion were assessed. Flap survival was assessed 7 days postoperatively.RESULTS:
Compared to the Control group, the RIPoC1 group showed statistically decreased MDA level at 6-, 12-, and 24-h reperfusion (P = 0.01, P < 0.01 and P < 0.01, respectively), and statistically increased SOD activity at 12- and 24-h reperfusion (P < 0.05 and P < 0.01, respectively). Flap survival rate on the 7th day was significantly higher in the RIPoC1 group than the control group (47.9 ± 6.4 vs . 29.4 ± 7.1 %, P < 0.01).CONCLUSION:
Three cycles of 5-min Limb remote ischemic post-conditioning rather than a single cycle of 15-min limb RIPoC has protective effect on flaps against ischemia-reperfusion injury by attenuating oxidative stress.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Lambeaux chirurgicaux
/
Lésion d'ischémie-reperfusion
/
Stress oxydatif
/
Postconditionnement ischémique
/
Procédures chirurgicales dermatologiques
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Acta cir. bras
Thème du journal:
Chirurgie générale
/
Procedimentos Cir£rgicos Operat¢rios
Année:
2016
Type:
Article
Pays d'affiliation:
Chine
Institution/Pays d'affiliation:
Shanghai Jiao Tong University/CN
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