COMPARISON OF PERMANENT STAINING METHODS FOR THE LABORATORY DIAGNOSIS OF TRICHOMONIASIS
Rev. Inst. Med. Trop. Säo Paulo
;
58: e5, 2016. tab
Article
Dans Anglais
| LILACS
| ID: lil-774573
ABSTRACT
Trichomonas vaginalis is the etiologic agent of trichomoniasis, the most common non-viral sexually transmitted disease (STD) in the world. The diagnosis is based on wet mount preparation and direct microscopy on fixed and stained clinical specimens. The aim of this study was to compare the performance of different fixing and staining techniques used in the detection of T. vaginalis in urine. The smears were fixed and submitted to different methods of permanent staining and then, the morphological aspects of the parasites were analyzed and compared. The Papanicolaou staining with ethanol as the fixative solution showed to be the best method of permanent staining. Our data suggest that staining techniques in association with wet mount examination of fresh specimens contribute to increase the sensitivity in the diagnosis of trichomoniasis.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Coloration et marquage
/
Trichomonase
/
Trichomonas vaginalis
Type d'étude:
Etude diagnostique
Limites du sujet:
Femelle
/
Humains
langue:
Anglais
Texte intégral:
Rev. Inst. Med. Trop. Säo Paulo
Thème du journal:
Médecine tropicale
Année:
2016
Type:
Article
/
descriptif de projet
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Universidade Federal do Rio Grande do Sul/BR
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