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Isoflavone formononetin from red propolis acts as a fungicide against Candida sp
Neves, Michelline Viviane Marques das; Silva, Tânia Maria Sarmento da; Lima, Edeltrudes de Oliveira; Cunha, Emídio Vasconcelos Leitão da; Oliveira, Eduardo de Jesus.
  • Neves, Michelline Viviane Marques das; Universidade Federal da Paraíba. Departamento de Biotecnologia. João Pessoa. BR
  • Silva, Tânia Maria Sarmento da; Universidade Federal da Paraíba. Departamento de Biotecnologia. João Pessoa. BR
  • Lima, Edeltrudes de Oliveira; Universidade Federal da Paraíba. Departamento de Biotecnologia. João Pessoa. BR
  • Cunha, Emídio Vasconcelos Leitão da; Universidade Federal da Paraíba. Departamento de Biotecnologia. João Pessoa. BR
  • Oliveira, Eduardo de Jesus; Universidade Federal da Paraíba. Departamento de Biotecnologia. João Pessoa. BR
Braz. j. microbiol ; 47(1): 159-166, Jan.-Mar. 2016. tab, graf
Article Dans Anglais | LILACS | ID: lil-775100
ABSTRACT
Abstract A bioassay-guided fractionation of two samples of Brazilian red propolis (from Igarassu, PE, Brazil, hereinafter propolis 1 and 2) was conducted in order to determine the components responsible for its antimicrobial activity, especially against Candida spp. Samples of both the crude powdered resin and the crude ethanolic extract of propolis from both locations inhibited the growth of all 12 tested Candida strains, with a minimum inhibitory concentration of 256 µg/mL. The hexane, acetate and methanol fractions of propolis 1 also inhibited all strains with minimum inhibitory concentration values ranging from 128 to 512 µg/mL for the six bacteria tested and from 32 to 1024 µg/mL for the yeasts. Similarly, hexane and acetate fractions of propolis sample 2 inhibited all microorganisms tested, with minimum inhibitory concentration values of 512 µg/mL for bacteria and 32 µg/mL for yeasts. The extracts were analyzed by HPLC and their phenolic profile allowed us to identify and quantitate one phenolic acid and seven flavonoids in the crude ethanolic extract. Formononetin and pinocembrin were the major constituents amongst the identified compounds. Formononetin was detected in all extracts and fractions tested, except for the methanolic fraction of sample 2. The isolated isoflavone formononetin inhibited the growth of all the microorganisms tested, with a minimum inhibitory concentration of 200 µg/mL for the six bacteria strains tested and 25 µg/mL for the six yeasts. Formononetin also exhibited fungicidal activity against five of the six yeasts tested. Taken together our results demonstrate that the isoflavone formononetin is implicated in the reported antimicrobial activity of red propolis.
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Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Propolis / Candida / Isoflavones / Anti-infectieux Pays comme sujet: Amérique du Sud / Brésil langue: Anglais Texte intégral: Braz. j. microbiol Thème du journal: Microbiologie Année: 2016 Type: Article Pays d'affiliation: Brésil Institution/Pays d'affiliation: Universidade Federal da Paraíba/BR

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Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Propolis / Candida / Isoflavones / Anti-infectieux Pays comme sujet: Amérique du Sud / Brésil langue: Anglais Texte intégral: Braz. j. microbiol Thème du journal: Microbiologie Année: 2016 Type: Article Pays d'affiliation: Brésil Institution/Pays d'affiliation: Universidade Federal da Paraíba/BR