Selection of reference genes for expression analysis in the entomophthoralean fungus Pandora neoaphidis
Braz. j. microbiol
;
47(1): 259-265, Jan.-Mar. 2016. tab, graf
Article
Dans Anglais
| LILACS
| ID: lil-775129
ABSTRACT
Abstract The selection of suitable reference genes is crucial for accurate quantification of gene expression and can add to our understanding of host–pathogen interactions. To identify suitable reference genes in Pandora neoaphidis, an obligate aphid pathogenic fungus, the expression of three traditional candidate genes including 18S rRNA(18S), 28S rRNA(28S) and elongation factor 1 alpha-like protein (EF1), were measured by quantitative polymerase chain reaction at different developmental stages (conidia, conidia with germ tubes, short hyphae and elongated hyphae), and under different nutritional conditions. We calculated the expression stability of candidate reference genes using four algorithms including geNorm, NormFinder, BestKeeper and Delta Ct. The analysis results revealed that the comprehensive ranking of candidate reference genes from the most stable to the least stable was 18S (1.189), 28S (1.414) and EF1 (3). The 18S was, therefore, the most suitable reference gene for real-time RT-PCR analysis of gene expression under all conditions. These results will support further studies on gene expression in P. neoaphidis.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Normes de référence
/
Entomophthorales
/
Analyse de profil d'expression de gènes
/
Réaction de polymérisation en chaine en temps réel
/
Gènes fongiques
Type d'étude:
Étude pronostique
langue:
Anglais
Texte intégral:
Braz. j. microbiol
Thème du journal:
Microbiologie
Année:
2016
Type:
Article
/
descriptif de projet
Pays d'affiliation:
Chine
Institution/Pays d'affiliation:
China Jiliang University/CN
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS