Ligninolytic fungus Polyporus sp. S133 mediated metabolic degradation of fluorene
Braz. j. microbiol
;
47(3): 610-616, July-Sept. 2016. tab, graf
Article
Dans Anglais
| LILACS
| ID: lil-788960
ABSTRACT
ABSTRACT This study aimed to investigate the impact of nonionic surfactants on the efficacy of fluorine degradation by Polyporus sp. S133 in a liquid culture. Fluorene was observed to be degraded in its entirety by Polyporus sp. S133 subsequent to a 23-day incubation period. The fastest cell growth rate was observed in the initial 7 days in the culture that was supplemented with Tween 80. The degradation process was primarily modulated by the activity of two ligninolytic enzymes, laccase and MnP. The highest laccase activity was stimulated by the addition of Tween 80 (2443 U/L) followed by mixed surfactant (1766 U/L) and Brij 35 (1655 U/L). UV-vis spectroscopy, TLC analysis and mass spectrum analysis of samples subsequent to the degradation process in the culture medium confirmed the biotransformation of fluorene. Two metabolites, 9-fluorenol (λmax 270, tR 8.0 min and m/z 254) and protocatechuic acid (λmax 260, tR 11.3 min and m/z 370), were identified in the treated medium.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Polyporus
/
Fluorènes
langue:
Anglais
Texte intégral:
Braz. j. microbiol
Thème du journal:
Microbiologie
Année:
2016
Type:
Article
Pays d'affiliation:
Malaisie
Institution/Pays d'affiliation:
Universiti Teknologi Malaysia/MY
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS