Strain specific variation of outer membrane proteins of wild Yersinia pestis strains subjected to different growth temperatures
Mem. Inst. Oswaldo Cruz
;
85(1): 107-11, jan.-mar. 1990. tab, ilus
Article
Dans Anglais
| LILACS
| ID: lil-85177
RESUMO
Three Yersinia pestis strains isolated from humans and one laboratory strain (EV76) were grown in rich media at 28§C and 37§C and their outer membrane protein composition compared by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-Page). Several proteins with molecular weights ranging from 34 kDa to 7 kDa were observed to change in relative abundance in samples grown at different temperatures. At least seven Y. pestis outer membrane proteins showed a temperature-dependent and strain-specific behaviour. Some differences between the outer membrane proteins of full-pathogenic wild isolates and the EV76 strain could aldso be detected and the relevance of this finding on the use of laboratory strains as a reference to the study of Y. pestis biological properties is discuted
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Protéines de la membrane externe bactérienne
/
Yersinia pestis
/
Membrane cellulaire
Limites du sujet:
Humains
Pays comme sujet:
Amérique du Sud
/
Brésil
langue:
Anglais
Texte intégral:
Mem. Inst. Oswaldo Cruz
Thème du journal:
Médecine tropicale
/
Parasitologie
Année:
1990
Type:
Article
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