Expression of an active foot-and-mouth disease virus RNA polymerase in Escherichia coli
Braz. j. med. biol. res
;
24(4): 359-63, 1991. ilus
Article
Dans Anglais
| LILACS
| ID: lil-99464
ABSTRACT
The expression of a native form of the foot-and-mouth disease virus RNA polymerase was obtained. Two oligonucleotides of 66 base pairs were used to renuild the 5' end of the gene and to introduce the first methionine codon. The expression of the active polymerase in E. coli was achieved by inserting the gene before the tac promoter of the pKK223-3 plasmid
Recherche sur Google
Indice:
LILAS (Amériques)
Sujet Principal:
DNA-directed RNA polymerases
/
Régulation de l'expression des gènes viraux
/
Escherichia coli
/
Syndrome mains-pieds-bouche
langue:
Anglais
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
Biologie
/
Médicament
Année:
1991
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS