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Heat shock transcription factors regulate heat induced cell death in a rat histiocytoma.
J Biosci ; 2007 Apr; 32(3): 585-93
Article Dans Anglais | IMSEAR | ID: sea-110749
ABSTRACT
Heat shock response is associated with the synthesis of heat shock proteins (Hsps) which is strictly regulated by different members of heat shock transcription factors (HSFs). We previously reported that a rat histiocytoma, BC-8 failed to synthesize Hsps when subjected to typical heat shock conditions (42 degrees C, 60 min). The lack of Hsp synthesis in these cells was due to a failure in HSF1 DNA binding activity. In the present study we report that BC-8 tumor cells when subjected to heat shock at higher temperature (43 degrees C, 60 min) or incubation for longer time at 42 degrees C, exhibited necrosis characteristics; however,under mild heat shock (42 degrees C, 30 min) conditions cells showed activation of autophagy. Mild heat shock treatment induced proteolysis of HSF1, and under similar conditions we observed an increase in HSF2 expression followed by its enhanced DNA binding activity. Inhibiting HSF1 proteolysis by reversible proteasome inhibition failed to inhibit heat shock induced autophagy. Compromising HSF2 expression but not HSF1 resulted in the inhibition of autophagy, suggesting HSF2 dependent activation of autophagy. We are reporting for the first time that HSF2 is heat inducible and functions in heat shock induced autophagic cell death in BC-8 tumor cells.
Sujets)
Texte intégral: Disponible Indice: IMSEAR (Asie du Sud-Est) Sujet Principal: Rats / Facteurs de transcription / Régulation de l'expression des gènes / Mort cellulaire / Lignée cellulaire tumorale / Protéines de liaison à l'ADN / Histiocytome / Température élevée / Protéines du choc thermique / Animaux langue: Anglais Texte intégral: J Biosci Année: 2007 Type: Article

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Texte intégral: Disponible Indice: IMSEAR (Asie du Sud-Est) Sujet Principal: Rats / Facteurs de transcription / Régulation de l'expression des gènes / Mort cellulaire / Lignée cellulaire tumorale / Protéines de liaison à l'ADN / Histiocytome / Température élevée / Protéines du choc thermique / Animaux langue: Anglais Texte intégral: J Biosci Année: 2007 Type: Article