The analysis of proteome changes in sunflower seeds induced by N+ implantation.
J Biosci
;
2006 Jun; 31(2): 247-53
Article
Dans Anglais
| IMSEAR
| ID: sea-111021
ABSTRACT
In this work, the proteomic changes induced by N+ ion implantation were investigated using a sunflower seed model by a two-dimensional electrophoretic analysis. To further understand the changes of total protein irradiated with N+ ion, a proteomic analysis of N+ ion implantation seeds was developed. Among approximately 369 total protein spots displayed in 2-D gels, eight specific proteins were found in non-implanted seeds while four proteins were found in implanted seeds. Six proteins were used for MALDI-TOF MS analysis, of which only two had been reported before. The proteins designated as No.29 showed 23.4% homology to MADS-box transcriptional factor HAM59, while No. 279 protein had 23.20% identity to homeobox-leucine zipper protein HAHB-4. The analysis of proteome changes induced by N+ implantation could provide a new clue to studying mutation mechanism of ion implantation. To our knowledge, this is the first report about the analysis of proteome changes induced by N+ implantation in sunflower seeds.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Protéines végétales
/
Graines
/
Données de séquences moléculaires
/
Électrophorèse bidimensionnelle sur gel
/
Séquence d'acides aminés
/
Spectrométrie de masse MALDI
/
Protéome
/
Helianthus
/
Azote
Type d'étude:
Étude pronostique
langue:
Anglais
Texte intégral:
J Biosci
Année:
2006
Type:
Article
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