An enzyme immunoassay for detection of Japanese encephalitis virus-induced chemotactic cytokine.
J Biosci
;
2000 Mar; 25(1): 47-55
Article
Dans Anglais
| IMSEAR
| ID: sea-111150
ABSTRACT
Japanese encephalitis virus (JEV) induces human peripheral blood monocytes to secrete a chemotactic cytokine [human macrophage-derived factor (hMDF)] which causes chemotaxis of neutrophils. The only known assay for hMDF cannot quantify its level in samples, so an enzyme immunoassay has been standardized for detection of hMDF and hMDF-specific antibodies in test samples. The reported enzyme linked immunosorbent assay (ELISA) was found to be sensitive (89%), specific (91%), accurate (92 2%) and reproducible and was able to detect a minimum concentration of 23 ng hMDF/ml in test samples. The chemotactic factor could be detected in JEV inoculated mouse sera and JEV infected culture fluids. Significant finding of the test was the detection of hMDF in sera of human cases of JE.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Humains
/
Test ELISA
/
Techniques immunoenzymatiques
/
Sensibilité et spécificité
/
Chimiokines CC
/
Virus de l'encéphalite japonaise (espèce)
/
Chimiokine CCL22
/
Animaux
/
Macrophages
/
Souris
Type d'étude:
Etude diagnostique
langue:
Anglais
Texte intégral:
J Biosci
Année:
2000
Type:
Article
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