Evidence for lipopolysaccharide-induced differentiation of RAW264.7 murine macrophage cell line into dendritic like cells.
J Biosci
;
2003 Feb; 28(1): 129-34
Article
Dans Anglais
| IMSEAR
| ID: sea-111192
ABSTRACT
Effect of lipopolysaccharide (LPS) on RAW264.7 macrophage cell line was studied. LPS-treated RAW264.7 cells increased in cell size and acquired distinct dendritic morphology. At the optimal dose of LPS (1 mg/ml), almost 70% RAW264.7 cells acquired dendritic morphology. Flow cytometric studies indicate that the cell surface markers known to be expressed on dendritic cells and involved in antigen presentation and T cell activation (B 7.1, B 7.2, CD40, MHC class II antigens and CD1d) were also markedly upregulated on LPS-treated RAW 264.7 cells. Our results suggest the possibility that LPS by itself could constitute a sufficient signal for differentiation of macrophages into DC-like cells.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Rats
/
Cellules dendritiques
/
Activation des lymphocytes
/
Lymphocytes T
/
Marqueurs biologiques
/
Antigènes d'histocompatibilité de classe II
/
Différenciation cellulaire
/
Régulation positive
/
Lignée cellulaire
/
Lipopolysaccharides
langue:
Anglais
Texte intégral:
J Biosci
Année:
2003
Type:
Article
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