Development and application of multiplex polymerase chain reaction for the etiological diagnosis of infectious endophthalmitis.
J Postgrad Med
;
2006 Jul-Sep; 52(3): 179-82
Article
Dans Anglais
| IMSEAR
| ID: sea-116192
ABSTRACT
BACKGROUND:
Uniplex polymerase chain reaction (PCR) for detection of bacterial and panfungal genome has been applied onto a large number of intraocular fluids facilitating management of infective endophthalmitis.AIM:
To develop and apply a novel, rapid multiplex polymerase chain reaction (mPCR) to detect the presence of eubacterial, Propionibacterium acnes and panfungal genomes in intraocular fluids from patients clinically diagnosed to have infective endophthalmitis. SETTINGS ANDDESIGN:
Prospective study. MATERIALS ANDMETHODS:
Conventional methods of direct microscopy by KOH/calcofluor mount, Gram's staining and culture were done on 30 (19 Aqueous humor-AH and 11 Vitreous fluid-VF) intraocular specimens and mPCR done for simultaneous detection of eubacterial, P. acnes and panfungal genomes.RESULTS:
mPCR detected an infectious etiology in 18 (60%) of 30 intraocular specimens. Eubacterial genome was detected in 12 (40%) specimens, P. acnes genome in 4 (13.3%) specimens and panfungal genome in 2 (6.6%) specimens. mPCR results correlated with those of uniplex PCR. mPCR results were available within 5-6 hours after receipt of specimen, as against 8 hours required for each uniplex PCR with three separate thermalcyclers for their completion. Consumption of Taq polymerase was reduced considerably for mPCR.CONCLUSION:
mPCR is a cost effective, single tube method for the simultaneous detection of eubacterial, P. acnes and panfungal genomes in intraocular specimens from patients with infective endophthalmitis. It is a more rapid procedure than uniplex PCRs and requires only a single thermalcycler.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Propionibacterium acnes
/
Humains
/
Candida albicans
/
Endophtalmie
/
Réaction de polymérisation en chaîne
/
Études prospectives
/
Sensibilité et spécificité
/
Génome fongique
/
Génome bactérien
/
Analyse coût-bénéfice
Type d'étude:
Etude diagnostique
/
Etude d'étiologie
/
Étude observationnelle
langue:
Anglais
Texte intégral:
J Postgrad Med
Année:
2006
Type:
Article
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