Diagnosis of human rotavirus in stool specimens: comparison of different methods.
Article
Dans Anglais
| IMSEAR
| ID: sea-1275
ABSTRACT
A total of 345 stool specimens of hospitalized children below 5 years of age with acute gastroenteritis were tested for the presence of rotavirus by Polyacrylamide gel electrophoresis (PAGE), a monoclonal antibody based enzyme immunoassay (EIA) and a latex agglutination test (LAT). Detection rate for PAGE and EIA were 24.9% (345/86) and 20.9% (345/70) respectively. Using PAGE as the standard, the sensitivity and specificity of EIA were 75.6% and 98.1% respectively. The sensitivity of LAT was 70.9% with 100% specificity (LAT was done in only PAGE positive specimens). LAT appeared as the simplest and economic for both bed side and field use.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Infections à rotavirus
/
Bangladesh
/
Femelle
/
Humains
/
Mâle
/
Nouveau-né
/
Tests au latex
/
Enfant d'âge préscolaire
/
Prévalence
/
Techniques immunoenzymatiques
Type d'étude:
Etude diagnostique
/
Étude de prévalence
Pays comme sujet:
Asie
langue:
Anglais
Année:
2006
Type:
Article
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