Rapid determination of nevirapine in human plasma by high- performance liquid chromatography.
Article
Dans Anglais
| IMSEAR
| ID: sea-136749
ABSTRACT
Objective:
To develop and validate a high-performance liquid chromatography (HPLC) method for the determination of nevirapine in human plasma.Methods:
A plasma sample and an internal standard were extracted with tert.-butyl methyl ether and determined nevirapine concentration by HPLC method. The limit of quantitation (LOQ), accuracy, precision, specificity, stability and recovery were tested for method validation.Results:
Standard curve was linear in the range 0.1 ตg/mL to 20 ตg/mL. The limit of quantitation was 0.1 ตg/ml. Coefficients of variation (CV) of intraday and inter-day precision were less than 4 %. Accuracy was range from 97-101%. The extraction recovery was range from 94-112 %.Conclusion:
A rapid, sensitive and specific HPLC method was developed and can be used for determination of plasma nevirapine concentration in adult and pediatric patients infected with HIV.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
langue:
Anglais
Année:
2007
Type:
Article
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