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Histological and radiographic evaluation of the muscle tissue of rats after implantation of bone morphogenic protein (rhBMP-2) in a scaffold of inorganic bone and after stimulation with low-power laser light.
Article Dans Anglais | IMSEAR | ID: sea-139893
ABSTRACT

Objective:

The present study histologically and radiologically evaluates the muscle tissue of rats after implantation of bone morphogenic protein (rhBMP-2) in a natural inorganic bone mineral scaffold from a bull calf femur and irradiation with low-power light laser. Materials and

Methods:

The right and left hind limbs of 16 rats were shaved and an incision was made in the muscle on the face corresponding to the median portion of the tibia, into which rhBMP-2 in a scaffold of inorganic bone was implanted. Two groups of limbs were formed control (G1) and laser irradiation (G2). G2 received diode laser light applied in the direction of the implant, at a dose of 8 J/cm2 for three minutes. On the 7th, 21st, 40th and 112th days after implantation, hind limbs of 4 animals were radiographed and their implants removed together with the surrounding tissue for study under the microscope. The histological results were graded as 0=absence, 1=slight presence, 2=representative and 3=very representative, with regard to the following events formation of osteoid structure, acute inflammation, chronic inflammation, fibrin deposition, neovascularization, foreign-body granuloma and fibrosis.

Results:

There were no statistically significant differences in these events at each evaluation times, between the two groups (P>0.05; Mann-Whitney test). Nevertheless, it could be concluded that the natural inorganic bone matrix with rhBMP-2, from the femur of a bull calf, is a biocompatible combination.

Conclusions:

Under these conditions, the inductive capacity of rhBMP-2 for cell differentiation was inhibited. There was a slight acceleration in tissue healing in the group that received irradiation with low-power laser light.
Sujets)
Implant résorbable; Animaux; Matériaux biocompatibles/usage thérapeutique; Trame osseuse/effets des médicaments et des substances chimiques; Trame osseuse/effets des radiations; Trame osseuse/transplantation; Protéine morphogénétique osseuse de type 2; Protéines morphogénétiques osseuses/administration et posologie; Protéines morphogénétiques osseuses/effets des radiations; Protéines morphogénétiques osseuses/usage thérapeutique; Bovins; Différenciation cellulaire/effets des médicaments et des substances chimiques; Différenciation cellulaire/effets des radiations; Fibrine/analyse; Fibrose; Granulome à corps étranger/étiologie; Granulome à corps étranger/anatomopathologie; Inflammation; Photothérapie de faible intensité/méthodes; Lasers à semiconducteur/usage thérapeutique; Mâle; Muscles squelettiques/anatomopathologie; Muscles squelettiques/imagerie diagnostique; Muscles squelettiques/chirurgie; Néovascularisation physiologique/effets des médicaments et des substances chimiques; Néovascularisation physiologique/effets des radiations; Ostéogenèse/effets des médicaments et des substances chimiques; Ostéogenèse/effets des radiations; Dose de rayonnement; Rats; Rat Wistar; Protéines recombinantes/administration et posologie; Protéines recombinantes/effets des radiations; Protéines recombinantes/usage thérapeutique; Facteurs temps; Structures d'échafaudage tissulaires; Facteur de croissance transformant bêta/administration et posologie; Facteur de croissance transformant bêta/effets des radiations; Facteur de croissance transformant bêta/usage thérapeutique; Cicatrisation de plaie/effets des médicaments et des substances chimiques; Cicatrisation de plaie/effets des radiations

Texte intégral: Disponible Indice: IMSEAR (Asie du Sud-Est) Sujet Principal: Ostéogenèse / Dose de rayonnement / Rats / Facteurs temps / Cicatrisation de plaie / Matériaux biocompatibles / Trame osseuse / Fibrose / Protéines recombinantes / Mâle langue: Anglais Année: 2009 Type: Article

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Texte intégral: Disponible Indice: IMSEAR (Asie du Sud-Est) Sujet Principal: Ostéogenèse / Dose de rayonnement / Rats / Facteurs temps / Cicatrisation de plaie / Matériaux biocompatibles / Trame osseuse / Fibrose / Protéines recombinantes / Mâle langue: Anglais Année: 2009 Type: Article