Presence of CTX gene cluster in environmental non-O1/O139 Vibrio cholerae and its potential clinical significance.
Indian J Med Microbiol
;
2012 Jul-Sept; 30(3): 285-289
Article
Dans Anglais
| IMSEAR
| ID: sea-143971
ABSTRACT
Purpose:
The aim of this study was to understand the epidemiological linkage of clinical and environmental isolates of Vibrio cholerae and to determine their genotypes and virulence genes content. Materials andMethods:
A total of 60 V. cholerae strains obtained from clinical specimens (n = 40) and surface waters (n = 20) were subjected to genotyping using PFGE and determination of their virulence-associated gene clusters.Result:
PCR analysis showed the presence of chromosomally located hly and RTX genetic elements in 100% and 90% of the environmental isolates, respectively. The phage-mediated genetic elements such as CTX, TLC and VPI were detected in 5% of the environmental isolates suggesting that the environmental isolates cannot acquire certain mobile gene clusters. A total of 4 and 18 pulsotypes were obtained among the clinical and environmental V. cholerae isolates, respectively. Non-pathogenic environmentally isolated V. cholerae constituted a distinct cluster with one single non-O1, non-O139 strain (EP6) carrying the virulence genes similar to the epidemic strains. This may suggest the possible potential of conversion of non-pathogenic to a pathogenic environmental strain.Conclusions:
The emergence of a single environmental isolate in our study containing the pathogenicity genes amongst the diverse non-pathogenic environmental isolates needs to be further studied in the context of V. cholerae pathogenicity sero-coversion.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Vibrio cholerae
/
Humains
/
Famille multigénique
/
Choléra
/
Toxine cholérique
/
Réaction de polymérisation en chaîne
/
Facteurs de virulence
/
Microbiologie de l'environnement
langue:
Anglais
Texte intégral:
Indian J Med Microbiol
Thème du journal:
Microbiology
Année:
2012
Type:
Article
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