Optimization of solid state fermentation conditions for the production of cellulase by Trichoderma reesei.

ABSTRACT

Cellulase production studies have been carried out using the fungal strain Trichoderma reesei NCIM 992 by using three different lignocellulosic materials by solid state fermentation (SSF). The effect of basic fermentation parameters (pH, temperature, moisture content, particle size of substrate and moistening agent) on enzyme production was studied. Maximum cellulase production was 2.63 U ml-1 using wheat bran as substrate. The optimal conditions for cellulase production for wheat bran were found to be initial moisture content-70%, initial medium pH-5.0, temperature-30oC, moistening agents (MSS) and particle size of substrate (500 @m).The optimal incubation time for production was six days. Results indicate the scope for further optimization of the production conditions to obtain higher cellulase titres using the strain under SSF.