In-vitro adhesion of Staphylococcus spp. to certain orthopedic biomaterials and expression of adhesion genes.

ABSTRACT

The present study evaluated biofilm forming capacity, the adherence of Staphylococci spp. to different orthopedic biomaterials and the presence of both icaA and icaD genes among staphylococci strains isolated from patients suffering from orthopedic implant infections. We studied 53 Staphylococcal strains from infections related to orthopedic implants, as regards their ability to form biofilm by using microtitre plate method (MTP), in vitro evaluation of the ability of the biofilm forming strains to adhere to certain biomaterials that used in orthopedic surgery and detection of ica A and ica D among the isolates. 90.9% of S. aureus strains were biofilm positive while, 95% of Coagulase negative staph. were biofilm forming, PMMA demonstrated a significantly highest adherence (P<0.05) followed by stainless steel while, the lowest adherence exhibited by titanium and Biofilm producing strains were positive for icaA and icaD genes while, biofilm negative strains were negative for both genes. Staphylococcus spp. are the major pathogens in orthopedic implants infections. Titanium biomaterials are less susceptible for adherence by bacteria . Biofilms are considered the key factor in the development of implantrelated infections.