Rapid and simple method of photobleaching to reduce background autofluorescence in lung tissue sections.
Indian J Biochem Biophys
;
2015 Feb; 52 (1): 107-110
Article
Dans Anglais
| IMSEAR
| ID: sea-157962
ABSTRACT
Autofluorescence exhibited by tissues often interferes with immunofluorescence. Using imaging and spectral analysis, we observed remarkable reduction of autofluorescence of formalin fixed paraffin embedded tissues irradiated with light prior to incubation with immunofluorescent dyes. The technique of photobleaching offers significant improvement in the quality and specificity of immunofluorescence. This has the potential for better techniques for disease diagnosis.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Spectrométrie de fluorescence
/
Anticorps antinucléaires
/
Technique d'immunofluorescence
/
Photoblanchiment
/
Poumon
langue:
Anglais
Texte intégral:
Indian J Biochem Biophys
Année:
2015
Type:
Article
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