Sequential release of cellulose enzymes during germination of Trichoderma reesei spores.

ABSTRACT

The pattern of release of extracellular cellulase during the germination of Trichoderma reesei spores has been examined. The four enzymes namely, filter paper degrading enzyme, β-1,4 endoglucanase, β-glucosidase and xylanase appear sequentially in the culture broth during germination of the spores. The order of enzyme appearance is not altered by the type of carbon source in the germination medium. Measureable quantities of filter paper degrading enzyme is detected only after the outgrowth has occurred. A possible mechanism of spore germination and induction of the enzymes by insoluble cellulose is suggested.