Cloning of ferredoxin I gene from Azotobacter vinelandii using synthetic oligonucleotide probes.

ABSTRACT

Two synthetic oligonucleotide probe mixtures, whose sequences were inferred from two separate stretches of amino acids, one closer to the carboxy terminal and the other closer to the amino terminal, of ferredoxin I protein of Azotobacter vinelandii, were used to select ferredoxin I gene clones from a cosmid gene library of Azotobacter vinelandii. Restriction analysis revealed that 7 out of 10 selected clones were of the same type. All these clones were found to hybridize with fixABCX genes of Rhizobium meliloti.