Physical, chemical parameter and optimization for the production of “moringa oleifera” enzyme extract: studies includes phytochemicals, minerals and antimicrobials.

ABSTRACT

Moringa Oleifera is one of the 14 species of family Moringaceae, native to India, Africa, Arabia, South Asia, South America and the pacific and Caribbean Islands. Moringa oleifera has been naturalized in many tropic and subtropic regions worldwide, the plant is referred by a number of names such as horseradish tree, drumstick tree, ben oil tree, miracle tree, and “Mothers best friend”. The present study is to investigate physical and chemical parameters on the production of enzyme from leaf extract of Moringa oleifera. The leaf extract of different solvents showed the enzyme activity of Protease and lipase. The enzymes were optimized at different temperatures, and different chemical and nitrogen sources. The enzyme activity was determined at the different time intervals. The extract of different solvents showed the antibacterial activity against the selective pathogens. The enzyme was electrophoresed in SDS PAGE and molecular weight was determined and reported.