Spectral Analysis and Chemical Studies of the Sweet Constituent, Rebaudioside A.
European J Med Plants
;
2012 Jan-Mar; 2(1): 57-65
Article
Dans Anglais
| IMSEAR
| ID: sea-163962
ABSTRACT
Aim:
To study NMR spectral analysis and hydrolysis products of Rebaudioside A (1) that has been isolated from Stevia rebaudiana. StudyDesign:
Acquiring NMR and high resolution mass spectral (HRMS) data of Rebaudioside A for structural characterization, and identification of hydrolysis products. Place of Study Organic Chemistry Department of The Coca-Cola Company, Atlanta, GA 30313, USA.Methodology:
The 1D (1H & 13C) and 2D (COSY, HSQC, and HMBC) NMR spectral data of was acquired using Varian Unity Plus 600 MHz instrument using standard pulse sequences. Hydrolysis studies were performed on Rebaudioside A using acid, base and enzymatic studies to identify partial degradation products, aglycone and sugar residues.Results:
The proton and carbon values of Rebaudioside A were assigned based on NMR and HRMS data as well as chemical studies. The aglycone was identified as steviol on the basis of enzymatic hydrolysis, sugar was identified as D-glucose from acid hydrolysis and the partial hydrolyzed product was identified as Rebaudioside B from alkaline hydrolysis.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
langue:
Anglais
Texte intégral:
European J Med Plants
Année:
2012
Type:
Article
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