Effect of aged garlic extract on immune responses to experimental fibrosarcoma tumor in BALB/c mice.
Indian J Cancer
;
2014 Oct-Dec; 51(4): 609-613
Article
Dans Anglais
| IMSEAR
| ID: sea-172584
ABSTRACT
BACKGROUND:
Aged garlic extract (AGE) has many biological activities including radical scavenging, antioxidative and immunomodulative effects.AIM:
In this research work, the antitumor and immunomodulatory effects of AGE against fibrosarcoma implanted tumor were studied. MATERIALS ANDMETHODS:
WEHI‑164 fibrosarcoma cells were implanted subcutaneously on day 0 into the right flank of 40 BALB/c mice at age of 8 weeks. Mice were randomly categorized in two separate groups First received AGE (100 mg/kg, IP), second group as the control group received phosphate buffered saline. Treatments were carried out 3 times/week. Tumor growth was measured and morbidity was recorded. Subpopulations of CD4+/CD8+ T cells were determined using flow cytometry. WEHI‑164 cell specific cytotoxicity of splenocytes and in vitro production of interferon gamma (IFN‑γ) and interleukin‑4 cytokines were measured.RESULTS:
The mice received AGE had significantly longer survival time compared with the control mice. The inhibitory effect on tumor growth was seen in AGE treated mice. The CD4+/CD8+ ratio and in vitro IFN‑γ production of splenocytes were significantly increased in AGE group. WEHI‑164 specific cytotoxicity of splenocytes from AGE mice was also significantly increased at 251 E T ratio.CONCLUSION:
Administration of AGE resulted in improved immune responses against experimentally implanted fibrosarcoma tumors in BALB/c mice. AGE showed significant effects on inhibition of tumor growth and longevity of survival times.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
langue:
Anglais
Texte intégral:
Indian J Cancer
Année:
2014
Type:
Article
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