Activity of Plumbago zeylanica Linn. root and Holoptelea integrifolia Roxb. bark pastes in acute and chronic paw infl ammation in Wistar rats.

ABSTRACT

Background: The pastes prepared from roots of Plumbago zeylanica Linn. and barks of Holoptelea integrifolia Roxb. are widely used by traditional healers for the treatment of arthritis in rural northern Karnataka. Objective: The present study was undertaken to scientifi cally evaluate the safety and effi cacy of traditionally used formulations in experimental animals. Materials and Methods: The study, approved by IAEC was carried out in male Wistar rats and dermal toxicity in rabbits. Carrageenan model was used to assess effect on acute infl ammation. Paw volume were measured at 1, 2, 4, and 6th hour postchallenge. Chronic infl ammation was developed by using Complete Freund’s Adjuvant (CFA). Paw volume, ankle joint circumference, and body weight were assessed on 1st, 4th, 8th, 14th, 17th, and 21st day. Paste was applied once every day to the infl amed area of the paw of respective groups of animals, continuously for 14 days. Statistics The data were analyzed by one way analysis of variance followed by Dunnett’s post hoc test. P ≤ 0.05 was considered as signifi cant. Results: The formulations did not show any dermal toxicity and found to be safe. Both the pastes signifi cantly (P < 0.05) suppressed, carrageenan-induced paw edema at 6th hour and Holoptelea integrifolia appears to be more effective than Plumbago zeylanica. Signifi cant reduction was observed in paw volume, ankle joint circumference and animal body weight gained. Conclusions: The tested formulations (P. zeylanica root and H. integrifolia bark pastes) showed signifi cant antiinfl ammatory activity. The present fi ndings therefore support its utility in arthritic pain, infl ammation and the claim of traditional practitioners.