Evaluation of a modified IRMA for anti-D quantitation, using 3H protein A.
Article
Dans Anglais
| IMSEAR
| ID: sea-20138
ABSTRACT
A modified immunoradiometry assay (IRMA) using tritiated (3H) protein A was developed to estimate anti-D concentration. The main advantages of the assay were longer shelf life of the labelled reagent (more than two years); minimum radiation hazard and; low non specific binding. Levels of anti-D were estimated in 23 Rh (D) immunised women. A good correlation of anti-D concentration (micrograms/ml) with Rh antibody titre was observed (r = +0.89, P < 0.01). Anti-D concentration estimated by 3H protein A IRMA correlated well with the severity of Rh-HDN. This assay could quantitate anti-D in sera having exclusively IgG3 subtype.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Système Rhésus
/
Iso-immunisation Rhésus
/
Protéine A staphylococcique
/
Tritium
/
Femelle
/
Humains
/
Immunoglobuline G
/
Test ELISA
/
Dosage radioimmunométrique
/
Alloanticorps
langue:
Anglais
Année:
1993
Type:
Article
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