Effect of a Siddha drug (Vatharasavangam) on insulin release from isolated pancreatic islets in rabbits.

ABSTRACT

The Siddha drug--Vatharasavangam (VRV) was studied in rabbits for its hypoglycemic activity. The insulin level in alloxan-induced hyperglycemic animals increased significantly after treatment with VRV. The insulin release from the isolated islets to glucose stimulus and acid phosphatase activity in islets as an index to islet function were measured. The insulin released from the islets in hyperglycemic rabbits decreased from 70 microU/ml (control) to 32 microU/ml to low glucose stimulus. VRV-treated rabbits showed a significant increase in the insulin released from the islet cells (65 microU/ml) to the low glucose stimulus. Similarly, a significant increase of insulin secretion was observed for high glucose stimulus after 30 min in VRV treated rabbits (100 microU/ml), when compared to hyperglycemic rabbits (70 microU/ml). The islet cell acid phosphatase activity in the hyperglycemic rabbits increased significantly after VRV treatment. These findings suggest that VRV acts by stimulating beta-cells to secrete insulin to the glucose signal and improves blood glucose homeostasis.