An Improved Lc-Ms/Ms Method Development And Validation For The Determination Of Trandolapril And Verapamil In Human Plasma
Int J Pharm Pharm Sci
;
2019 Mar; 11(3): 91-95
Article
| IMSEAR
| ID: sea-205862
ABSTRACT
Objective:
A simple, sensitive and rapid LC-MS/MS technique was developed for the quantitation of trandolapril (TDL) and verapamil (VPL) in a biological matrix and validated.Methods:
Sample preparation processed by SPE (Solid Phase extraction) on phenomenex cartridge using Ledipasvir as an internal standard. Two drugs were eluted on waters symmetry-RP18 (5µ, 150 mm×4.0 mm) column with the mobile composition of 10 mmol ammonium formate and ACN(acetonitrile) in the ratio of 7030 %V/V. Detection and quantitation were processed by electrospray ionization in positive ionization mode.Results:
The quantification approach was validated in 5-1500 ng/ml linear concentration range for TDL and 1-2000 ng/ml for VPL. The intraday and inter-day precision and accuracy were found to be 0.58% to 5.69% and 93% to 104% for two drugs. The average recoveries for TDL and VPL were found to be 92.9% and 93.5% respectively.Conclusion:
The developed work was validated and can be applicable to the routine analysis of TDL and VPL simultaneously in a biological matrix.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Texte intégral:
Int J Pharm Pharm Sci
Année:
2019
Type:
Article
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