Identification of TNF-Þ-308G/A (rsl800629) polymorphism in Bangladeshi population related to type-2 diabetes

ABSTRACT

This study was designed to inspect the association between tumor necrosis factor (TNF)-α-308G/A (rsl800629)polymorphism with diabetes mellitus type-2 (DMT2) in Bangladeshi population. Besides, the role ofTNF-α-308G/A in early proliferation of DMT2 has been investigated. The allelic frequency of TNF-α-308G/Aand their association with DMT2 was studied using high resolution melting (HRM) analysis and confirmedusing sequencing. A few demographic risk factors associated with DMT2 were also investigated routinely.The significance of these risk factors was analyzed statistically. We have analyzed 657 individuals who weredistributed into two groups 330 non-diabetic controls and 327 DMT2 individuals. HRM analysis showsthat 11 individuals bare G/A and 2 bare A/A genotype in DMT2 patients. Within non-diabetic individual,we found only one with G/A genotype. The frequency of TNF-α-308G>A are within the Hardy–Weinbergequilibrium (0.00482) at 95% confidence level. TNF-α-308G>A frequency in two age group based on firsttime diagnosed, we found association with early proliferation of DMT2 with a p-value of 0.008965 in Fisher’stest at 95% confidence level. Our result suggested that the single nucleotide polymorphisms TNF-α-308G>Ais closely associated with DMT2 patients in Bangladeshi population. Besides, presence of TNF-α-308G>Apolymorphism increases the risk of early proliferation of DMT2.