Screening of cytotoxic activities toward WiDr and Vero cell lines of ethyl acetate extracts of fungi-derived from the marine sponge Acanthostrongylophora ingens

ABSTRACT

Ethyl acetate extracts of fungi-derived from the marine sponge Acanthostrongylophora ingens were tested forcytotoxic activity against WiDr and Vero cell lines. Three of fungi extracts exhibited strong cytotoxicity with percentageof viability (≤50%) occurring at concentrations of 100 µg/ml. One isolate (IB141) showed specific cytotoxicityagainst WiDr cells whreas not against Vero cells. This isolate was identified based on molecular characterizationusing sequence analysis of the partial 18S rRNA gene. The result indicated that IB141 was identical to Aspergillusochraceus. A comparatively high part of positive bio-activity screening results were acquired in this study, displayingthat the fungi-derived from the marine sponge A. ingens have potential as a source of new anti-cancer agents.