Antibody response to outer membrane proteins in Shigella dysenteriae type 1 infection with special reference to appearance of murine antibodies.

ABSTRACT

In shigellosis, the protective immune mechanism is not well established. The bacterial outer membrane proteins (OMPs) may have a role in the induction of immunity due to their outwardly location. The serum antibody response of S. dysenteriae type 1 infected patients against OMPs was assessed by enzyme linked immunosorbent assay (ELISA). A striking elevation of serum IgG response was noted during the convalescent phase. Murine antiserum directed against S. dysenteriae 1 OMPs was found to be highly cross reactive with the OMPs isolated from heterologous species. A major antigenic OMP was partially purified and showed distinct immunodominance in ELISA. These observations suggest that the specific component may have some immunoprophylactic potential.