Alterations in brain tumor DNA detected by a fingerprinting probe.
Indian J Biochem Biophys
;
1996 Dec; 33(6): 455-7
Article
Dans Anglais
| IMSEAR
| ID: sea-26769
ABSTRACT
We used a novel DNA fingerprinting probe O-chi-1 (ref. 1) to detect differences in the hybridization pattern of brain tumor DNA and paired normal tissue of a given individual. Representatives of meningiomas (two), glioblastoma multeforme (three) and astrocytoma (one) were studied. Alterations, which included amplification as well as the loss of a normal band in tumor DNA, were observed in four of the six tumours. While the increased intensity of a band can be taken to imply increased copy number, the disappearance of bands could either be due to loss of DNA sequence or rearrangement resulting in different sized bands.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Astrocytome
/
Tumeurs du cerveau
/
Humains
/
ADN tumoral
/
Type II site-specific deoxyribonuclease
/
Sondes d'ADN
/
Technique de Southern
/
Profilage d'ADN
/
Glioblastome
/
Électrophorèse sur gel d'agar
langue:
Anglais
Texte intégral:
Indian J Biochem Biophys
Année:
1996
Type:
Article
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