Ascorbate-dependent formation of hydroxyl radicals in the presence of iron chelates.
Indian J Biochem Biophys
;
1993 Oct; 30(5): 289-92
Article
Dans Anglais
| IMSEAR
| ID: sea-26876
ABSTRACT
The autoxidation of L-ascorbate on incubation in saline phosphate buffer (pH 7.4) is accompanied by hydroxyl radical (.OH) generation. The metal chelator EDTA showed significant inhibition of ascorbate autoxidation and ascorbate-dependent .OH release. On the other hand, Fe2+ (EDTA) greatly augmented both ascorbate autoxidation and ascorbate-dependent .OH production. The biological iron chelating compounds such as ATP, ADP, citrate and pyrophosphate suppressed both ascorbate autoxidation and ascorbate-mediated .OH production, thereby indicating that these compounds suppress the activating effect of iron. Ascorbate autoxidation and ascorbate-dependent .OH formation, stimulated by Fe2+ (EDTA) were significantly inhibited by .OH scavengers, namely mannitol, thiourea and sodium formate, as well as by catalase and to a lesser extent by bovine serum albumin, superoxide dismutase (native and heat denatured) and heat denatured catalase.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Oxydoréduction
/
Acide ascorbique
/
Superoxide dismutase
/
Thiourée
/
Cinétique
/
Composés du fer II
/
Catalase
/
ADP
/
Adénosine triphosphate
/
Agents chélateurs du fer
langue:
Anglais
Texte intégral:
Indian J Biochem Biophys
Année:
1993
Type:
Article
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