Biochemical and dynamic studies of collagen from human normal skin and keloid tissue.
Indian J Biochem Biophys
;
1999 Jun; 36(3): 158-64
Article
Dans Anglais
| IMSEAR
| ID: sea-27419
ABSTRACT
Keloids are exuberant scars, in which collagen, fibronectin and glycosaminoglycans are overdeposited. Biochemical analysis of the collagen isolated from normal skin and keloid tissue by pepsin treatment, indicated an increase in the type III and GAG content. Viscosity measurements of collagen from normal skin and keloid tissue were used in the present study to establish the interaction between collagen and GAG. Physico-chemical properties such as intrinsic viscosity, reduced viscosity and hydrated volume were computed from viscosity measurements. These measurements were also used to determine the denaturation temperature of collagen which was further confirmed by DSC measurements. Chondroitinase has been used in this study to probe the influence of GAG on the physico-chemical characteristic of keloid collagen.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Peau
/
Viscosité
/
Humains
/
Collagène
/
Électrophorèse sur gel de polyacrylamide
/
Glycosaminoglycanes
/
Température élevée
/
Chéloïde
langue:
Anglais
Texte intégral:
Indian J Biochem Biophys
Année:
1999
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS