Stabilization of prostaglandin synthetase by immobilization of goat seminal microsomes on silica gel-G.
Indian J Biochem Biophys
;
1990 Apr; 27(2): 76-80
Article
Dans Anglais
| IMSEAR
| ID: sea-28845
ABSTRACT
Prostaglandin synthetase was immobilized by adsorption of goat vesicular microsomes on silica gel containing CaSO4 (silica gel G). Repeated cycles of enzymatic conversion of arachidonic acid to prostaglandin by the immobilized microsomes increased the product yield by 1.5 fold, in comparison to the same by free microsomal particles. The presence of Ca2+ in silica gel is responsible for this improved yield of prostaglandin as the divalent metal ion stabilized prostaglandin synthetase activity in a remarkable way. Microsomal particles immobilized on solid supports like alumina G and controlled pore glass were not very effective.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Sperme
/
Stabilité enzymatique
/
Mâle
/
Capra
/
Prostaglandin-endoperoxide synthases
/
Silice
/
Enzymes immobilisées
/
Gels
/
Animaux
/
Microsomes
langue:
Anglais
Texte intégral:
Indian J Biochem Biophys
Année:
1990
Type:
Article
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