Isatin (2,3-dioxoindole): a competitive inhibitor of Na(+)-dependent lysine uptake in rat instestine.
Indian J Biochem Biophys
;
1996 Dec; 33(6): 519-22
Article
Dans Anglais
| IMSEAR
| ID: sea-28963
ABSTRACT
Isatin (2,3-dioxoindole) competitively inhibited (27-40%) Na(+)-dependent L-lysine uptake in rat intestine. The value of Kt was increased from 3.04 mM in control to 5.88 mM in presence of 10 mM isatin. Effect of isatin on the Na(+)-independent amino acid uptake was insignificant (12-18%). The inhibitory constant (Ki) was 2.8 mM under these conditions. The observed inhibition was unaffected by -SH group reacting agents. Isatin (1-10 mM) inhibited Na+, K(+)-ATPase activity in intestine in vitro, the maximum inhibition (66%) being at 10 mM isatin concentration. But the drug had no effect on enzyme activity under in vivo conditions.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Rats
/
Sodium
/
Réactifs sulfhydryle
/
Transport biologique
/
Cinétique
/
Sodium-Potassium-Exchanging ATPase
/
Muqueuse intestinale
/
Isatine
/
Animaux
/
Lysine
langue:
Anglais
Texte intégral:
Indian J Biochem Biophys
Année:
1996
Type:
Article
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