Ca(2+)-induced alterations in the activity of membrane (Ca(2+)-Mg2+)-ATPase of human and rat erythrocytes.
Indian J Biochem Biophys
;
1993 Apr; 30(2): 98-102
Article
Dans Anglais
| IMSEAR
| ID: sea-29045
ABSTRACT
Effect of intraerythrocyte Ca2+ elevation on human and rat erythrocyte membrane (Ca(2+)-Mg2+)-ATPase along with that of incubation of the erythrocyte ghosts in their own hemolysates enriched with Ca2+ has been studied. While the membrane (Ca(2+)-Mg2+)-ATPase levels of Ca(2+)-loaded human erythrocytes showed an initial increase and subsequent decline, membranes incubated in their own hemolysate showed a consistent decrease in the enzyme activity. Calmodulin sensitivity was retained by the preparations in contrast to the earlier observations made with washed erythrocyte membranes. Similar changes in (Ca(2+)-Mg2+)-ATPase activity but of greater magnitude were observed in response to Ca2+ in the calpain-rich rat erythrocytes. Considerable crosslinking and proteolysis was observed in case of human and rat erythrocytes exposed to high Ca2+ concentrations. The Ca(2+)-activable transglutaminase, however, did not play any role in the activation of the (Ca(2+)-Mg2+)-ATPase.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Rats
/
Humains
/
Calcium
/
Ca(2/) Mg(2/)-ATPase
/
Activation enzymatique
/
Membrane érythrocytaire
/
Animaux
/
Lipides membranaires
langue:
Anglais
Texte intégral:
Indian J Biochem Biophys
Année:
1993
Type:
Article
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