Hormonal modulation of secretion of immunoreactive riboflavin carrier protein by adult rat Leydig cells in vitro.
Indian J Biochem Biophys
;
1996 Aug; 33(4): 274-80
Article
Dans Anglais
| IMSEAR
| ID: sea-29099
ABSTRACT
Adult rat Leydig cells in culture synthesize and secrete riboflavin carrier protein (RCP) as demonstrated by [35S]-methionine incorporation into newly synthesized proteins followed by immunoprecipitation as well as specific radioimmunoassay. LH stimulates the secretion of RCP 4-fold which could be inhibited upto 75% by an aromatase inhibitor. 8-bromo-cyclic AMP and cholera toxin could mimic the LH stimulated secretion of the carrier protein. The extent of stimulation of RCP secretion brought about by exogenous estradiol-17 beta is comparable to that of LH. The antiestrogen tamoxifen, when added along with either LH or estrogen, inhibited the stimulated levels significantly. These results show that the estrogen-inducible riboflavin carrier is secreted by Leydig cells under positive regulation of LH.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Protéines de transport membranaire
/
Rats
/
Riboflavine
/
Tamoxifène
/
8-Bromo AMP cyclique
/
Mâle
/
Hormone lutéinisante
/
Protéines de transport
/
Rat Wistar
/
Oestradiol
langue:
Anglais
Texte intégral:
Indian J Biochem Biophys
Année:
1996
Type:
Article
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