Detection of blood stage antigens of Plasmodium vivax by sandwich ELISA using pan-species monoclonal antibodies and polyclonal antibodies.
Southeast Asian J Trop Med Public Health
;
1991 Sep; 22(3): 393-6
Article
Dans Anglais
| IMSEAR
| ID: sea-30709
ABSTRACT
This paper reports an improved PcAb-McAb-ELISA test to detect blood stage Plasmodium vivax antigen in which the plates were coated with rabbit anti-P. cynomolgi polyclonal antibody to capture the antigens in test samples and two monoclonal antibodies, M26-32 and 3F9, were added together to react with the captured antigens. The coincidence rate with this test was 93% with microscopically confirmed P. vivax cases, 97% with normal samples, 95% with microscopically negative fever cases from nonendemic areas and 86% from endemic areas, respectively. The sensitivity was greater than 1 parasite/10(5) RBC.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Plasmodium vivax
/
Humains
/
Test ELISA
/
Anticorps antiprotozoaires
/
Sensibilité et spécificité
/
Paludisme à Plasmodium vivax
/
Études d'évaluation comme sujet
/
Faux négatifs
/
Faux positifs
/
Microscopie
Type d'étude:
Etude diagnostique
/
Études d'évaluation
langue:
Anglais
Texte intégral:
Southeast Asian J Trop Med Public Health
Année:
1991
Type:
Article
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