Detection of filarial antigen using antibodies raised against Wuchereria bancrofti microfilarial SDS soluble antigen.
Southeast Asian J Trop Med Public Health
;
1992 Sep; 23(3): 444-9
Article
Dans Anglais
| IMSEAR
| ID: sea-32673
ABSTRACT
Polyclonal antibodies raised in mouse ascitic fluid against Wuchereria bancrofti microfilarial antigens (Wb Mf SDS S Ag) were studied for their diagnostic use in bancroftian filariasis using a dip stick, enzyme-linked immunosorbent assay. In sandwich ELISA, 100% of microfilaremic sera (30 out of 30) 53% of acute filarial sera (7/13), 40% of subacute filarial sera (6 out of 15), 13% of chronic filarial sera (2/15) and 20% of endemic area normal sera (3/15) showed the presence of filarial antigen. Determination of filarial antigen titer in microfilaremic sera showed an apparent positive correlation between microfilarial density and antigen titer. The antibody raised against Wb Mf SDS S Ag was found to be cross reactive with phosphorylcholine epitopes. The filarial antigen detected by anti Wb Mf SDS S Ag antibodies in sandwich ELISA is possibly associated with the active stage (microfilaremia) of infection.
Texte intégral:
Disponible
Indice:
IMSEAR (Asie du Sud-Est)
Sujet Principal:
Solubilité
/
Wuchereria bancrofti
/
Humains
/
Test ELISA
/
Anticorps antihelminthe
/
Filarioses
/
Animaux
/
Souris
/
Souris de lignée BALB C
/
Microfilaria
Type d'étude:
Etude diagnostique
langue:
Anglais
Texte intégral:
Southeast Asian J Trop Med Public Health
Année:
1992
Type:
Article
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